Step 1. Restriction Enzyme Digestion

Step 2. Gel Electrophoresis

Step 3. DNA DenaturationStep 4. Transfer DNA to Membrane

Step 5. Making a Probe

Step 6. Pre-hybridization

Step 7. Hybridization

Step 8. Washes

Step 9. Anti-DIG

Step 9. Anti-DIG

Step 10. Washes

Step 11. CSPD

Step 12. Detection